1,137
edits
Changes
Jump to navigation
Jump to search
Line 14:
Line 14: − + + + + + + + + + + + + + + + + + + + + + + + + + + +
→Rat c-fos Primers: added our current primers


==Rat c-fos Primers==
==Rat c-fos Primers used by Alcala and Houpt==
Forward:
Reverse:
Product size:
PCR conditions for use with HotStarTaq mix (progam CFOSGENO on thermocycler)
95° for 15 min to activate Taq
Repeat 35 times:
:94° for 1 min
:59° for 1 min
:72° for 1 min
:72° for 10 min final extension
4° for overnight
==Rat c-fos Primers from Literature==
From Shimizu et al., Cancer Sci 2007; 98: 1318–1322
From Shimizu et al., Cancer Sci 2007; 98: 1318–1322