Line 14:
Line 14:


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==Rat c-fos Primers==
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==Rat c-fos Primers used by Alcala and Houpt==
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Forward:
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Reverse:
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Product size:
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PCR conditions for use with HotStarTaq mix (progam CFOSGENO on thermocycler)
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95° for 15 min to activate Taq
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Repeat 35 times:
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:94° for 1 min
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:59° for 1 min
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:72° for 1 min
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:72° for 10 min final extension
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4° for overnight
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==Rat c-fos Primers from Literature==
From Shimizu et al., Cancer Sci 2007; 98: 1318–1322
From Shimizu et al., Cancer Sci 2007; 98: 1318–1322