Changes

C-Fos Methylation (view source)

321 bytes added , 16:23, 23 January 2009

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==Rat c-fos Primers used by Alcala and Houpt==

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Primers designed with Primer 3 using the 300 bases upstream of c-Fos transcription start based on gi|62750806|ref|NC_005105.2|NC_005105

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(equivalent to bases 172-471 of U02631, which claims start of 5’UTR is at 455.)

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Forward:

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Forward at -261 (cFosSense-261):

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:5'-CCATCCCCGAAATCCTACAT-3'

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Reverse:

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Reverse at -6 (cFosAS-6):

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:5'-CAATCGCGGTTGGAGTAGTA-3'

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Product size:

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Product size: 256 bp

PCR conditions for use with HotStarTaq mix (progam CFOSGENO on thermocycler)

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4° for overnight

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==Rat c-fos Primers from Literature==

1,145

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