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JA Lateral Hypothalamus Lesions (view source)
Revision as of 11:20, 12 May 2011
, 11:20, 12 May 2011added quinine
'''2. Test Conditioned Taste Aversion (2 x 2'''3 days)
'''2. Test Conditioned Taste Aversion (2 x 23 days)'''
:Rats on 8 days of water restriction (3 h, 3h, 1h, 1h, 30 min, 30 min, 10 min , 10 min)
:Rats on 8 days of water restriction (3 h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min)
:Conditioning day: 10 min access to 0.125% saccharin (CS)
:Conditioning day: 10 min access to 0.125% saccharin (CS)
'''3. Histology to Confirm Lesion (1 week)'''
'''3. Test response to aversive taste (2 days)'''
:Run 2-bottle test of water vs. quinine HCl solution to demonstrate ability to reject aversive taste.
'''4. Histology to Confirm Lesion (1 week)'''
:Perfuse rats and dissect brains.
:Perfuse rats and dissect brains.