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JA Lateral Hypothalamus Lesions (view source)
Revision as of 09:03, 19 May 2011
, 09:03, 19 May 2011added Norgren lesion parameters
'''1. Lesions of Lateral Hypothalamus (8 days)'''
'''1. Lesions of Lateral Hypothalamus (8 days)'''
From Touzani and Scalfani 2002 PMID 12492437
:The rats were held in a stereotaxic apparatus with the incisor bar set 3.3 mm below the interaural line. An injection cannula (outer diameter = 0.24 mm) was positioned in the LH at coordinates 6.0 mm anterior to the interaural line, 1.7 mm lateral to the sagittal suture and 9.0 mm ventral from the skull surface.
:The rats were held in a stereotaxic apparatus with the incisor bar set 3.3 mm below the interaural line. An injection cannula (outer diameter = 0.24 mm) was positioned in the LH at coordinates 6.0 mm anterior to the interaural line, 1.7 mm lateral to the sagittal suture and 9.0 mm ventral from the skull surface.
:5 mg of ibotenic acid (Sigma Chemical, St. Louis, MO, USA) in 0.5 uL of vehicle (phosphate buffer, pH 7.2).
:5 ug of ibotenic acid (Sigma Chemical, St. Louis, MO, USA) in 0.5 uL of vehicle (phosphate buffer, pH 7.2).
:Each injection lasted 6.5 min and 10 more min elapsed before removal of the cannula.
From Dayawansa et al. PMID 21270347 (Norgren Lab)
:Bilateral LH lesions (LHx) positioned stereotaxically at -3.0 mm from bregma, 2.0 mm lateral, and 8.8 mm deep to the skull surface.
:10 ug of ibotenic acid (0.5 ul, 20 ug/ul in PBS, pH 7.4; Research Biochemicals, Natick, MA).
:Each injection occurred over 25 min via the needle of a 1.ul Hamilton syringe. Infusions were made manually.
:Allow 1 week for recovery.
:Allow 1 week for recovery.
'''4. Test in conditioned flavor preference?'''
'''4. Histology to Confirm Lesion (1 week)'''
'''5. Histology to Confirm Lesion (1 week)'''
:Perfuse rats and dissect brains.
:Perfuse rats and dissect brains.