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JA Lateral Hypothalamus Lesions
(view source)
Revision as of 11:20, 12 May 2011
157 bytes added
,
11:20, 12 May 2011
added quinine
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'''2. Test Conditioned Taste Aversion (2 x
2
'''
3 days)
+
'''2. Test Conditioned Taste Aversion (2 x
23 days)
'''
−
:Rats on 8 days of water restriction (3 h, 3h, 1h, 1h, 30 min, 30 min, 10 min , 10 min)
+
:Rats on 8 days of water restriction (3 h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min)
:Conditioning day: 10 min access to 0.125% saccharin (CS)
:Conditioning day: 10 min access to 0.125% saccharin (CS)
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Line 30:
−
'''3. Histology to Confirm Lesion (1 week)'''
+
'''3
. Test response to aversive taste (2 days)'''
+
+
:Run 2-bottle test of water vs. quinine HCl solution to demonstrate ability to reject aversive taste.
+
+
+
+
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'''4
. Histology to Confirm Lesion (1 week)'''
:Perfuse rats and dissect brains.
:Perfuse rats and dissect brains.
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