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Each rat was stereotaxically implanted with a 22-gauge, stainless steel, guide
Each rat was stereotaxically implanted with a 22-gauge, stainless steel, guide
cannula (Plastics One, Roanoke, VA) aimed towards the lateral cerebral ventricle (1.2 mm caudal to bregma, 1.5 mm
cannula (model C313G, Plastics One, Roanoke, VA) aimed towards the lateral cerebral ventricle (1.2 mm caudal to bregma, 1.5 mm
lateral to the midline, and 4 mm below the skull surface; Houpt 1998 PMID 9437750). Guide cannulas were held in place with dental acrylic bonded to
lateral to the midline, and 4 mm below the skull surface; Houpt 1998 PMID 9437750). Guide cannulas were held in place with dental acrylic bonded to
stainless steel screws anchored to the skull. An obdurator was inserted into each guide cannula and remained in place
stainless steel screws anchored to the skull. A 28-gauge obdurator was inserted into each guide cannula and remained in place
except during injections when it was removed and replaced with an injector that extended 1.0 mm beyond the tip of the guide cannula.
except during injections when it was removed and replaced with an 28-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
===Test===
===Test===