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DMC Sodium Butyrate and Flavor Conditioning (view source)

Revision as of 09:51, 17 October 2019

897 bytes added ,  09:51, 17 October 2019
fleshed out agenda
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Test if systemic Sodium Butyrate, an HDAC inhibitor, enhances preference learning
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==People==
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Days 1-7: Weigh rats daily. Food and water restricted: water for 30 min, food 3-4 pellets to maintain at 90% body weight.
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Day 8: Conditioning Day 2
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Durba Mukherjee, Alex Haimovich
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-15 min: (check time and dose!) inject with NaCl or sodium butyrate (0.3 M, 12 ml/kg, i.p.)
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0 min: CS+/8% glucose
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30 min: remove solution, give food pellets
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Day 9: Conditioning Day 2
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==Agenda==
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-15 min: (check time!) inject with NaCl or sodium butyrate
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0 min: CS-/saccharin
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30 min: remove solution, return food and water ad lib
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Days 10-17: 2 bottle preference tests of CS+/sac and CS-/sac
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n=16 adult male sprague-dawley rats (Charles River CD), individually housed in BRF 169
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<br>
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'''Days 0-8: Food and water restricted.''' (October 8 - October 16, 2019)
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Weigh rats daily.  water for 30 min, food 3-4 pellets to maintain at 90% body weight.
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Water removed on Tuesday Oct 8, 2019. 30 min water at 1pm daily, pellet ration at 1:30pm
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<br>
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'''Day 9: Conditioning Day 1''' (October 17, 2019)
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0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape)
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30 min: remove CS+, inject with 3ml/kg of 0.3M sodium butyrate or 3m/kg of 0.15M NaCl, give daily pellet ration
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* Rats 1-8: grape CS+, Rats 9-16: Cherry CS+.
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* Odd rats: NaCL injection. Even rats: NaB injection
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<br>
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'''Day 10: Conditioning Day 1''' (October 18, 2019)
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0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape)
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30 min: remove CS-, inject with 3ml/kg of 0.3M sodium butyrate or 3m/kg of 0.15M NaCl, return food and water ad lib
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* Rats 1-8: Cherry CS-, Rats 9-16: Grape CS-.
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* Odd rats: NaCL injection. Even rats: NaB injection
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<br>
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'''Days 11-17: 2 bottle preference tests''' (October 19-25, 2019)
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Each rat given adlib 24-h access to of CS+/sac vs CS-/sac.  Position of bottle alternated each day.
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[[Category:Flavor Preference]]
 
[[Category:Flavor Preference]]
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