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Line 1: + − + − Days 1-7: Weigh rats daily. Food and water restricted: water for 30 min, food 3-4 pellets to maintain at 90% body weight. − Day 8: Conditioning Day 2+ − -15 min: (check time and dose!) inject with NaCl or sodium butyrate (0.3 M, 12 ml/kg, i.p.) − 0 min: CS+/8% glucose − 30 min: remove solution, give food pellets − Day 9: Conditioning Day 2+ − -15 min: (check time!) inject with NaCl or sodium butyrate − 0 min: CS-/saccharin − 30 min: remove solution, return food and water ad lib − Days 10-17: 2 bottle preference tests of CS+/sac and CS-/sac+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
DMC Sodium Butyrate and Flavor Conditioning (view source)
Revision as of 09:51, 17 October 2019
, 09:51, 17 October 2019fleshed out agenda
Test if systemic Sodium Butyrate, an HDAC inhibitor, enhances preference learning
==People==
Durba Mukherjee, Alex Haimovich
==Agenda==
n=16 adult male sprague-dawley rats (Charles River CD), individually housed in BRF 169
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'''Days 0-8: Food and water restricted.''' (October 8 - October 16, 2019)
Weigh rats daily. water for 30 min, food 3-4 pellets to maintain at 90% body weight.
Water removed on Tuesday Oct 8, 2019. 30 min water at 1pm daily, pellet ration at 1:30pm
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'''Day 9: Conditioning Day 1''' (October 17, 2019)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape)
30 min: remove CS+, inject with 3ml/kg of 0.3M sodium butyrate or 3m/kg of 0.15M NaCl, give daily pellet ration
* Rats 1-8: grape CS+, Rats 9-16: Cherry CS+.
* Odd rats: NaCL injection. Even rats: NaB injection
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'''Day 10: Conditioning Day 1''' (October 18, 2019)
0 min: 30 min access to CS+ 005% Kool-Aid + 0.05% saccharin + 8% glucose (counterbalanced cherry, grape)
30 min: remove CS-, inject with 3ml/kg of 0.3M sodium butyrate or 3m/kg of 0.15M NaCl, return food and water ad lib
* Rats 1-8: Cherry CS-, Rats 9-16: Grape CS-.
* Odd rats: NaCL injection. Even rats: NaB injection
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'''Days 11-17: 2 bottle preference tests''' (October 19-25, 2019)
Each rat given adlib 24-h access to of CS+/sac vs CS-/sac. Position of bottle alternated each day.
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[[Category:Flavor Preference]]
[[Category:Flavor Preference]]