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Methylation-Sensitive Enzymes (view source)

Revision as of 14:40, 4 July 2022

8 bytes added ,  14:40, 4 July 2022
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Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.
 
Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.
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Enzyme NEB link Site Cut Position Reaction Conditions
 
Enzyme NEB link Site Cut Position Reaction Conditions
 
HpaII https://www.neb.com/products/r0171-hpaii CCgg 5’...C/CGG...3'<br>3’...GGC/C...5’ 1X NEBuffer 1, Incubate at 37°C.
 
HpaII https://www.neb.com/products/r0171-hpaii CCgg 5’...C/CGG...3'<br>3’...GGC/C...5’ 1X NEBuffer 1, Incubate at 37°C.
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