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Methylation-Sensitive Enzymes (view source)
Revision as of 16:19, 7 August 2017
, 16:19, 7 August 2017deleted quotes
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Enzyme NEB link site Cut Position Reaction Conditions
Enzyme NEB link Site Cut Position Reaction Conditions
HpaII https://www.neb.com/products/r0171-hpaii CCgg "5’...C/CGG...3'<br>3’...GGC/C...5’" 1X NEBuffer 1, Incubate at 37°C.
HpaII https://www.neb.com/products/r0171-hpaii CCgg 5’...C/CGG...3'<br>3’...GGC/C...5’ 1X NEBuffer 1, Incubate at 37°C.
MspI https://www.neb.com/products/r0106-mspi CCgg "5’...C/CGG...3'<br>3’...GGC/C...5’" 1X NEBuffer 4, Incubate at 37°C.
MspI https://www.neb.com/products/r0106-mspi CCgg 5’...C/CGG...3'<br>3’...GGC/C...5’ 1X NEBuffer 4, Incubate at 37°C.
AciI https://www.neb.com/products/r0551-aciip CCgC "5’...C/CGC...3'<br>3’...GGC/G...5’" 1X NEBuffer 3, Incubate at 37°C.
AciI https://www.neb.com/products/r0551-aciip CCgC 5’...C/CGC...3'<br>3’...GGC/G...5’ 1X NEBuffer 3, Incubate at 37°C.
HhaI https://www.neb.com/products/r0139-hhai gCgC "5’...GCG/C...3'<br>3’...C/GCG...5’" 1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C.
HhaI https://www.neb.com/products/r0139-hhai gCgC 5’...GCG/C...3'<br>3’...C/GCG...5’ 1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C.
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[[Category:Protocols]]
[[Category:Protocols]]