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Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.
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Enzyme NEB link site Cut Position Reaction Conditions
HpaII https://www.neb.com/products/r0171-hpaii CCgg "5’...C/CGG...3'<br>3’...GGC/C...5’" 1X NEBuffer 1, Incubate at 37°C.
MspI https://www.neb.com/products/r0106-mspi CCgg "5’...C/CGG...3'<br>3’...GGC/C...5’" 1X NEBuffer 4, Incubate at 37°C.
AciI https://www.neb.com/products/r0551-aciip CCgC "5’...C/CGC...3'<br>3’...GGC/G...5’" 1X NEBuffer 3, Incubate at 37°C.
HhaI https://www.neb.com/products/r0139-hhai gCgC "5’...GCG/C...3'<br>3’...C/GCG...5’" 1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C.
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[[Category:Protocols]]
Methylation-Sensitive Enzymes (view source)
Revision as of 17:18, 7 August 2017
, 17:18, 7 August 2017created page of methylation sensitive DNA restriction enzymes
Enzymes which do NOT cut DNA if there is a methylated cytosine in the restriction site.
<tab class="wikitable" border = "1" cellspacing="3" cellpadding = "3" head = top>
Enzyme NEB link site Cut Position Reaction Conditions
HpaII https://www.neb.com/products/r0171-hpaii CCgg "5’...C/CGG...3'<br>3’...GGC/C...5’" 1X NEBuffer 1, Incubate at 37°C.
MspI https://www.neb.com/products/r0106-mspi CCgg "5’...C/CGG...3'<br>3’...GGC/C...5’" 1X NEBuffer 4, Incubate at 37°C.
AciI https://www.neb.com/products/r0551-aciip CCgC "5’...C/CGC...3'<br>3’...GGC/G...5’" 1X NEBuffer 3, Incubate at 37°C.
HhaI https://www.neb.com/products/r0139-hhai gCgC "5’...GCG/C...3'<br>3’...C/GCG...5’" 1X NEBuffer 4, Supplemented with 100 μg/ml Bovine Serum Albumin, Incubate at 37°C.
</tab>
[[Category:Protocols]]