Changes

4 groups:

4 groups:

1. Isotonic LiCl and 4-OHT, N=6

1. Isotonic LiCl and 4-OHT, N=6: Excitatory DREADDs are expected to be expressed

2. Isotonic LiCl and  sesame oil, N=6

2. Isotonic LiCl and  sesame oil, N=6: No DREADDs are expected to be expressed

3. Isotonic saline and 4-OHT, N=6

3. Isotonic saline and 4-OHT, N=6: Due to the presence of 4-OHT but no LiCl, some DREADD expression is expected

4. Isotonic saline and sesame oil, N=5

4. Isotonic saline and sesame oil, N=5 No DREADD expression expected

'''Day 12: Conditioning Day''' (2023-9-24)

'''Day 12: Conditioning Day''' (2023-9-24)

Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min

Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p). ***Only LVL group will receive LiCl (0.15M,20ml/kg, i.p) after 10 min  

Groups:  

Groups:  

* LTL: Exp group (predicted to show attenuated taste aversion)

* LTL: Exp group (Predicted to form taste aversion)

* LVL: + Control (expect to form taste aversion)

* LVL: + Control (expect to form taste aversion)

* VTV: - Control (expect to not form taste aversion)

* VTV: - Control (expect to not form taste aversion)

* JCE01-04: Female

* JCF01-04: ???

* JCE05-08: Female

* JCF05-08: ???

* JCE09-12: Male

* JCF09-12: ???

* JCE13-16: Male

* JCF13-16: ???

After injection, ad lib water returned overnight.

After injection, ad lib water returned overnight.