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Line 37: − + − + − + − − 3. Isotonic saline and 4-OHT, N=6: Due to the presence of 4-OHT but no LiCl, some DREADD expression is expected − − 4. Isotonic saline and sesame oil, N=5 No DREADD expression expected Line 64:
Line 60: − + − + − + − * VTV: - Control (expect to not form taste aversion) − * VVV: - Control (expect to not form taste aversion)
JCF Excitatory DREADD Functional Validation (view source)
Revision as of 18:02, 27 January 2024
, 27 January→Agenda
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Pre Treatment (1/27/24) 2 weeks before starting water restriction
Pre Treatment (1/28/24) 2 weeks before starting water restriction
4 groups:
4 groups:
1. Isotonic LiCl and 4-OHT, N=6: Excitatory DREADDs are expected to be expressed
1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed
2. Isotonic LiCl and sesame oil, N=6: No DREADDs are expected to be expressed
2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed
'''Day 12: Conditioning Day''' (2023-9-24)
'''Day 12: Conditioning Day''' (2023-9-24)
Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p). ***Only LVL group will receive LiCl (0.15M,20ml/kg, i.p) after 10 min
Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 10 min
Groups:
Groups:
* LTL: Exp group (Predicted to form taste aversion)
* LTC: Exp group (Predicted to form taste aversion)
* LVL: + Control (expect to form taste aversion)
* VVC: Control (expect to not form taste aversion)