Changes

Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.  

Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.  

Pre Treatment (1/27/24) 2 weeks before starting water restriction

Pre Treatment (1/28/24) 2 weeks before starting water restriction

4 groups:

4 groups:

1. Isotonic LiCl and 4-OHT, N=6: Excitatory DREADDs are expected to be expressed

1. Isotonic LiCl and 4-OHT, N=12: Excitatory DREADDs are expected to be expressed

2. Isotonic LiCl and sesame oil, N=6: No DREADDs are expected to be expressed

2. Isotonic Saline and sesame oil, N=11: No DREADDs are expected to be expressed

 

 

'''Day 12: Conditioning Day''' (2023-9-24)

'''Day 12: Conditioning Day''' (2023-9-24)

Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p). ***Only LVL group will receive LiCl (0.15M,20ml/kg, i.p) after 10 min  

Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) after 10 min  

Groups:  

Groups:  

* LTL: Exp group (Predicted to form taste aversion)

* LTC: Exp group (Predicted to form taste aversion)

* LVL: + Control (expect to form taste aversion)

* VVC: Control (expect to not form taste aversion)       

* VVV: - Control (expect to not form taste aversion)