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Fix perm redirect
=Primers for Davenport's Real-Time PCR experiment=
=Primers for Davenport's Real-Time PCR experiment=
Primers were ordered 2009 March from Sigma Aldrich
Primers were ordered 2009 May from Sigma Aldrich
(designed using [https://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi PrimerPlus3])
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Probe Abbr Sequence Tm Size Genbank Reference
Rat NR1 subunit QNR1FOR ttcacagaagtgcgatctgg 60.0 108 U11418.1,2360-2467 PrimerPlus
QNR1REV atggacagggaaacgttctg 60.0
Rat Serine Racemase QSERRACFOR tggtagatgcgttggtggta 60.0 103 NM_198757, 856-958 PrimerPlus
QSERRACREV tcagcagcgtacaccttcac 60.1
Rat GADPH QGADPHFOR tcactgccactcagaagactg 60 123 DQ403053, 467-589 Andre designed with sequencher
QGADPHREV ggatgaccttgcccacagc 60 . . .
</tab>
Primers were ordered 2009 March from Sigma Aldrich (NB: these primers worked fine in a test RT-PCR, but Andre said the product sizes should be 100-150bp, and the Tm's should be closer together. So, we redesigned and ordered primers above).
<tab class="wikitable" border = "1" cellspacing="3" cellpadding = "3" head = top>
<tab class="wikitable" border = "1" cellspacing="3" cellpadding = "3" head = top>
Note: GADPHFOR and REV maybe mixed up . . . .
Note: GADPHFOR and REV maybe mixed up . . . .
</tab>
</tab>
=Primers for Real-Time PCR=
=Primers for Real-Time PCR=
Looked at primers from Yamamoto et al. 2008 PMID 17957211 using Applied Biosystems, but ended up designing own primers above.
[[Category:Taste_Aversion]]
[[Category:Taste_Aversion]]