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SR in situ probe
=Serine Racemase Probe for in situ hybridization=
Primer sequences taken from Yoshikawa et al., PMID 16973158
Rat serine racemase. Genbank Ascension # NM_198757
PCR product Length: 295 bases (+22 bases for T7 promoter]
Forward Primer 664 to 683
ATT GCA AGA AAC TGG CCA TC
Reverse Primer [with T7 promoter] 958 to 939
[CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC
PCR amplification conditions (from Yoshikawa et al, PMID 15337321):
10-min predenaturation at 95 °C
15-s denaturation at 95 °C.
20-s at 58 °C for serine racemase
followed by a 20-s extension at 72 °C.
Primer sequences taken from Yoshikawa et al., PMID 16973158
Rat serine racemase. Genbank Ascension # NM_198757
PCR product Length: 295 bases (+22 bases for T7 promoter]
Forward Primer 664 to 683
ATT GCA AGA AAC TGG CCA TC
Reverse Primer [with T7 promoter] 958 to 939
[CTT AAT ACG ACT CAC TAT AGG G] TC AGC AGC GTA CAC CTT CAC
PCR amplification conditions (from Yoshikawa et al, PMID 15337321):
10-min predenaturation at 95 °C
15-s denaturation at 95 °C.
20-s at 58 °C for serine racemase
followed by a 20-s extension at 72 °C.