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Line 13:
Line 13: − + − + − + − +
→Serine Racemase Probe for in situ hybridization
PCR amplification conditions (from Yoshikawa et al, PMID 15337321):
PCR amplification conditions (from Yoshikawa et al, PMID 15337321):
10-min predenaturation at 95 °C
*10-min predenaturation at 95 °C
15-s denaturation at 95 °C.
*15-s denaturation at 95 °C.
20-s at 58 °C for serine racemase
*20-s at 58 °C for serine racemase
followed by a 20-s extension at 72 °C.
*followed by a 20-s extension at 72 °C.