Changes

[CTT AAT ACG ACT CAC TAT  AGG G] TC AGC AGC GTA CAC CTT CAC

[CTT AAT ACG ACT CAC TAT  AGG G] TC AGC AGC GTA CAC CTT CAC

PCR amplification conditions (from Yoshikawa et al, PMID 15337321):  

*PCR amplification conditions (from Yoshikawa et al, PMID 15337321):  

*10-min predenaturation at 95 °C

**10-min predenaturation at 95 °C

*15-s denaturation at 95  °C.

**15-s denaturation at 95  °C.

*20-s at 58 °C for serine racemase

**20-s at 58 °C for serine racemase

*followed by a 20-s extension at 72  °C.

**followed by a 20-s extension at 72  °C.