Changes

PCR product Length: 295 bases (+22 bases for T7 promoter]

PCR product Length: 295 bases (+22 bases for T7 promoter]

Forward Primer 664 to 683  

Forward Primer 664 to 683  

ATT GCA AGA AAC TGG CCA TC

:ATT GCA AGA AAC TGG CCA TC

Reverse Primer [with T7 promoter]  958 to 939

Reverse Primer [with T7 promoter]  958 to 939

[CTT AAT ACG ACT CAC TAT  AGG G] TC AGC AGC GTA CAC CTT CAC

:[CTT AAT ACG ACT CAC TAT  AGG G] TC AGC AGC GTA CAC CTT CAC

*PCR amplification conditions (from Yoshikawa et al, PMID 15337321):  

Amplification conditions (from Yoshikawa et al, PMID 15337321):  

**10-min predenaturation at 95 °C

:10-min predenaturation at 95 °C

**15-s denaturation at 95  °C.

:15-s denaturation at 95  °C.

**20-s at 58 °C for serine racemase

:20-s at 58 °C for serine racemase

**followed by a 20-s extension at 72  °C.

:followed by a 20-s extension at 72  °C.