Changes

<b>MK-801: </b> Hasimoto et al. PMID 17109841 used qRT-PCR and HPLC to detect changes in SR and DAAO in adult rat brain after acute or chronic injections of MK-801, the non-competitive NR antagonist. Changes in mRNA were only seen at 400 ug/kg and above, Very large changes (5x - 10x at 1 h, still elevated 2-3x at 4 h) were seen in all brain regions examined (striatum hippocampys, cortex, diencephalon, midbrain, pons/medulla and cerebellum (although absolute levels were lower in more caudal brain regions.)  DAAO mRNA levels were decreased by MK-801 at 1 h, but increased at 4 h (and absolute levels of DAAO mRNA were high in more caudal brain regions.) DAAO showed a more dose-dependent response.  Chronic MK-801 (400 ug/kg for 14 days) also increased SR mRNA (2x in forebrain) but did not affect DAAO expression.  There is little discussion of the possible mechanisms, aside from pointing out that MK-801 can induce c-fos and there is an AP-1 element in the SR promoter, and  a CRE element in the DAAO promoter.

<b>MK-801: </b> Hasimoto et al. PMID 17109841 used qRT-PCR and HPLC to detect changes in SR and DAAO in adult rat brain after acute or chronic injections of MK-801, the non-competitive NR antagonist. Changes in mRNA were only seen at 400 ug/kg and above, Very large changes (5x - 10x at 1 h, still elevated 2-3x at 4 h) were seen in all brain regions examined (striatum hippocampys, cortex, diencephalon, midbrain, pons/medulla and cerebellum (although absolute levels were lower in more caudal brain regions.)  DAAO mRNA levels were decreased by MK-801 at 1 h, but increased at 4 h (and absolute levels of DAAO mRNA were high in more caudal brain regions.) DAAO showed a more dose-dependent response.  Chronic MK-801 (400 ug/kg for 14 days) also increased SR mRNA (2x in forebrain) but did not affect DAAO expression.  There is little discussion of the possible mechanisms, aside from pointing out that MK-801 can induce c-fos and there is an AP-1 element in the SR promoter, and  a CRE element in the DAAO promoter.

<b>MK-801: </b>

<b>MK-801: </b> Yoshikawa et al. PMID 15337321 using qt-PCR showed an increase in SR mRNA at 2-6h after systemic administration of MK-801 (0.4 mg/kg). Very nice description of qRT-PCR controls and calibration.

<b>Ketamine</b> Takeyama et al. PMID 16716293

<b>Ketamine</b> Takeyama et al. PMID 16716293 using qRT-PCR showed an increase in SR mRNA and DAAO at 4 h (but not 2 h or 6h) after systemic ketamine (50 mg/kg) A dose response curve at the 4 h time point showed a dose dependent increse in SR and DAAO at 50 and 100 mg/kg

 

<b>Beta-Amyloid</b> Wu et al. PMID 15285800

<b>Beta-Amyloid</b> Wu et al. PMID 15285800

<b>ischemia</b> Wang and Zhu PMID 15066209

<b>ischemia</b> Wang and Zhu PMID 15066209