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Line 12: − + − + − Look up references by Leo the olfactory fellow from NIH+ − '''Doublecortin''' − marker for migrating cells DCX (Santa Cruz) From Imayoashi et al PMID 18758458+ − + + + + + + + + + + + +
added ordering info
=Useful Markers=
=Useful Markers=
'''Ki67'''
=='''Ki67''' ==
marker for mitotic cells
Ki67 is a marker for proliferating cells, because it is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0) PMID 10653597 .
a rabbit anti-rat Ki67 monoclonal antibody (1:750, Biomeda, Foster City, CA) From Corniola et al PMID 18778998
a rabbit anti-rat Ki67 monoclonal antibody (1:750, Biomeda, Foster City, CA) From Corniola et al PMID 18778998
'''GAD67'''
=='''GAD67'''==
anti-GAD67 rabbit antibody (1/1,000 for Alexa 594-conjugated streptavidin detection, 1/500,000 for TSA detection; (Chemicon) From Tamamaki N et al PMID 14574680
anti-GAD67 rabbit antibody from Chemicon. Used by Tamamaki N et al to confirm expression pattern of GAD67-GFP mice PMID 14574680 Used by Stewart et al to demonstate GAD/GABA expression in SVZ progenitor cells PMID 11891665.
Millipore (nee Chemicon) Catalog # [http://www.millipore.com/catalogue/item/ab9706 AB9706] , Anti-GAD67 affinity purified rabbit polyclonal antibody; described for Western blotting. 100 µg, $239
=='''Doublecortin'''==
'''NeuroTrace'''
DCX is a marker for migrating cells DCX (Santa Cruz) From Imayoashi et al PMID 18758458
Santa Cruz Catalog # [http://www.scbt.com/datasheet-8066-doublecortin-c-18-antibody.html sc-8066] anti-DCX goat polyclonal antibody. 200 µg/ml, $259
Found a posting on the interweb that recommended (instead of Santa Cruz) Abcam's anti-DCX antiserum [http://www.abcam.com/index.html?datasheet=18723 ab18723], might try that for $325. Requires a heat retrieval step by heating at 60C.
=='''NeuroTrace'''==
Molecular Probes from Bagley et al PMID 17996088
Molecular Probes from Bagley et al PMID 17996088
Invitrogen NeuroTrace 435/455 blue fluorescent Nissl stain catalog #[http://products.invitrogen.com/ivgn/en/US/adirect/invitrogen?cmd=catProductDetail&entryPoint=adirect&messageType=catProductDetail&showAddButton=true&productID=N21479 N-21479] 1 ml, $120
Mount Neurotrace with [http://www.invitrogen.com/site/us/en/home/brands/Molecular-Probes/Key-Molecular-Probes-Products/ProLong-Antifades-Brand-Page.html Prolong Gold AntiFade] invitrogen Catalog P36930 10 ml, $114.00