Changes

[[Image:sgal.tiff|400px|left]]

[[Image:sgal.png|400px|left]]

Image from Heuermann and Cosgrove PMID 11355350

Image from Heuermann and Cosgrove PMID 11355350

S-Gal, IPTG and ferric ammonium citrate are dry-blended with standard LB agar at the final concentrations stated above. The powdered blend was suspended in deionized water and autoclaved as previously described. For the preparation of microwaved medium, the suspended blend was heated until boiling, followed by swirling to allow the remaining agar component to go into solution. In this case, the antibiotic ampicillin was added after autoclaving (100 mg/mL).  

S-Gal, IPTG and ferric ammonium citrate are dry-blended with standard LB agar at the final concentrations stated above. The powdered blend was suspended in deionized water and autoclaved as previously described. For the preparation of microwaved medium, the suspended blend was heated until boiling, followed by swirling to allow the remaining agar component to go into solution. In this case, the antibiotic ampicillin was added after autoclaving (100 mg/mL).

 

=X-Gal=

=X-Gal=