Changes

β-D-galactopyranoside, C19H21O9Na, MW 416.35,  MDL Number MFCD03458481.

β-D-galactopyranoside, C19H21O9Na, MW 416.35,  MDL Number MFCD03458481.

S-Gal®, sodium salt is a patented water-soluble, autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. Caution: For black color development to occur, ferric ion must be present. Although we recommend ferric ammonium citrate (500 mg/L of media), other ferric compounds can be used to provide this requirement, depending upon your particular system.  From [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S7313%7CSIGMA&N5=SEARCH_CONCAT_PNO%7CBRAND_KEY&F=SPEC Sigma-Aldrich site].

S-Gal®, sodium salt is a patented water-soluble, autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. Caution: For black color development to occur, ferric ion must be present. Although we recommend ferric ammonium citrate (500 mg/L of media), other ferric compounds can be used to provide this requirement, depending upon your particular system.  From [http://www.sigmaaldrich.com/catalog/product/sigma/s7313?lang=en&region=US Sigma-Aldrich site].

==S-Gal staining conditions==

==S-Gal staining conditions==

According to [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=S7313%7CSIGMA&N5=SEARCH_CONCAT_PNO%7CBRAND_KEY&F=SPEC Sigma-Aldrich site]:  

According to [http://www.sigmaaldrich.com/catalog/product/sigma/s7313?lang=en&region=US Sigma-Aldrich site]:  

Stock solutions of S-Gal can be made by dissolving at 50 mg/mL in deionized water, sterile-filtering and storing at -20 °C.

Stock solutions of S-Gal can be made by dissolving at 50 mg/mL in deionized water, sterile-filtering and storing at -20 °C.