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DMK Valproate and Saccharin CTA 2 (view source)
Revision as of 11:19, 26 August 2021
, 11:19, 26 August 2021updated page with dates and schedule
==Data==
[https://houptlab.org/bartab/expt/?id=DMK Raw Data Link]
==People==
Durba Mukherjee
==CTA Conditioning==
===Agenda===
'''Rats arrive''' ( , 2021)
n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
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'''Day 0: Water removed''' ( July 14, 2021)
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'''Days 1 - 7: Water Restriction''' ( July 15 - 21 , 2021)
Weigh rats daily. Water for 30 min, ad lib food.
30 min water at 1430 daily.
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'''Day 8: Conditioning Day''' (July 22 2021)
-15 min: half of rats injected with 0.15M Na Valproate 8ml/kg = 200mg/kg; other half 0.15M NaCl 8ml/kg
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip.
ad lib water returned after final injection overnight.
* rats 1-8 : male, Rats 9-16: female
* rats 1-4, 9-12: Valproate; rats 5-8,13-16 NaCl
* All rats: saccharin
* odd rats: 3ml/kg of 0.15M LiCl; odd rats: 3ml/kg of 0.15M NaCl
'''Days 9 - 20: Preference Tests''' (July 23 - August 4, 2021)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 12 days.
[[Category:Flavor Preference]]
[[Category:Taste Aversion]]