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Repeat of Valproate-enhanced LiCl-CTA in male and female rats.
{{Infobox experiment|align=right
| Name = Valproate 100 mg/kg Enhanced CTA pt2
| project = HDAC Sex Difference
| code = DMS
| protocol = 1917
| funding = Chair's Fund
| investigators = Mukherjee, Houpt
| species = rat
| date = 2022-1-15
| type = CTA
| drugs = valproate, lithium
| artifacts =
| data = https://houptlab.org/bartab/expt/?id=DMS
}}
==Data==
==Data==
===Agenda===
===Agenda===
'''Rats arrive''' (?????)
'''Rats arrive(?????)'''
Sprague Dawley rats (dob ????? ), arrived
Sprague Dawley rats (dob ????? )
'''Day 0: Water removed 2022-1-15'''
<br>
'''Days 1-8: Water Restriction 2022-1-16 thru 2022-1-23'''
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
<br>
'''Day 9: Conditioning Day 2022-24'''
* -15 min: 8 ml/kg (100 mg/kg) of Valproate 75mm
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg ip of 0.15M LiCl or NaCl
Groups:
* DMS01-04 Val-Li-Male
* DMS05-08 Val-NaCl-Male
* DMS09-12 Val-Li-Female
* DMS13-16 Val-NaCl-Female
ad lib water returned after final injection overnight.
<br>
'''Days 10-21: Preference Tests: 2022-1-25 to 2022-2-6'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for 12 days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.