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added 488, and vectashield links
Here are the immunofluorescence filters that we probably need.
Here are the immunofluorescence filters that we probably need. Bolded are the fluorophores we frequently use.
<tab sep=tab border=1>
<tab sep=tab border=1>
Probe Excitation (nm) Emission (nm) Color
Probe Excitation (nm) Emission (nm) Color
Neurotrace 435 455 blue
Neurotrace 435 455 blue
DAPI 358 461 blue
DAPI 358 461 blue (often use mixed in [https://vectorlabs.com/products/vectashieldr-plus-antifade-mounting-medium-dapi VectaShield])
'''DyLight 405''' 400 421 blue
'''DyLight 405''' 400 421 blue
Brilliant Violet 421 407 421 blue (expensive)
Brilliant Violet 421 407 421 blue (expensive)
'''GFP''' 425 500 green
'''GFP''' 425 500 green
'''Fluorescein (FITC)''' 492 520 green
'''Alexa Fluor 488''' 493 519 green
Fluorescein (FITC) 492 520 green
YFP 500 535 yellow
YFP 500 535 yellow
Cyanine Cy3 550 570 orange
Cyanine Cy3 550 570 orange
Alexa Fluor 647 red, not for mercury lamps
Alexa Fluor 647 red, not for mercury lamps
</tab>
</tab>
[https://vectorlabs.com/products/vectashield-plus-antifade VECTASHIELD® PLUS Antifade Mounting Medium (H-1900)]
[https://vectorlabs.com/products/vectashieldr-plus-antifade-mounting-medium-dapi VECTASHIELD® PLUS Antifade Mounting Medium with DAPI (H-2000)]