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Make mixtures of LiCl, Li<sub>2</sub>CO<sub>3</sub>, and LiOr and several "physiological" solutions. Measure free Li+ with electrode. For Tris and plasma, also measure osmolality.
Make mixtures of LiCl, Li<sub>2</sub>CO<sub>3</sub>, and LiOr and several "physiological" solutions. Measure free Li+ with electrode. For Tris and plasma, also measure osmolality.
===1. Tris===
===Tris===
Tris-LiCl, Tris-LiOH, Tris-LiOH-Orotate (across concentration and pH ranges)
Tris-LiCl, Tris-LiOH, Tris-LiOH-Orotate (across concentration and pH ranges)
===2. Simulated Gastric Fluid (SGF)===
===Simulated Gastric Fluid (SGF)===
Li+ electrode cannot function at low pH (~2.0) of SGF, so need to neutralize mixtures with sodium bicarbonate (NaCH0<sub>3</sub>). Assay free Li+ when added before or after neutralization.
Li+ electrode cannot function at low pH (~2.0) of SGF, so need to neutralize mixtures with sodium bicarbonate (NaCH0<sub>3</sub>). Assay free Li+ when added before or after neutralization.
SGF-LiCl -> + NaHC0<sub>3</sub>, SGF-Tris-LiOr -> + NaHC0<sub>3</sub>, SGF -> + NaHC0<sub>3</sub> + LiCl, SGF -> + NaHC03 + Tris-LiOr
SGF-LiCl -> + NaHC0<sub>3</sub>, SGF-Tris-LiOr -> + NaHC0<sub>3</sub>, SGF -> + NaHC0<sub>3</sub> + LiCl, SGF -> + NaHC03 + Tris-LiOr
===3. Rat plasma===
===Rat plasma===
Blood taken from terminal cardiac puncture, heparinized, plasma separated and frozen at -20° C.
Blood taken from terminal cardiac puncture, heparinized, plasma separated and frozen at -20° C.
plasma-LiCl, plasma-Li<sub>2</sub>CO<sub>3</sub>, plasma-LiOr
plasma-LiCl, plasma-Li<sub>2</sub>CO<sub>3</sub>, plasma-LiOr
===4. Artificial Urine (AU)===
===Artificial Urine (AU)===
Artificial urine prepared according to DIN EN 1616:1999, from Pickering Laboratories (Mountain View, USA).
Artificial urine prepared according to DIN EN 1616:1999, from Pickering Laboratories (Mountain View, USA).