1,114
edits
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cleaned up
Line 2:
Line 2:
Taken from Yamamoto et al. 2008 PMID 17957211 using Applied Biosystems+
−
−NR1
− Forward primer 5'-GTTCTTCCGCTCAGGCTTTG-3'
− Reverse primer 5'-AGGGAAACGTTCTGCTTCCA-3'
− TaqMan probe 5'-FAM-CGGCATGCGCAAGGACAGCC-TAMRA-3'
−
−NR2A
− Forward primer 5'-AGCCCCCTTCGTCATCGTA-3'
− Reverse primer 5'-GACAGGGCACCGTGTTCCT-3'
− TaqMan probe 5'-FAM-AGGACATAGACCCCCTGACTGAGACCTGTG-TAMRA-3'
−
−NR2B
− Forward primer 5'-CCCCCAAGTTCTGGTTGGT-3'
− Reverse primer 5'-TTTTGGGAACGAGCTTTGCT-3'
− TaqMan probe 5'-FAM-TTGGCCGTCTTGGCCGTATCAGG-TAMRA-3'
−
−NR2C
− Forward primer 5'-GGGTGATGATGTTCGTGATGTG-3'
− Reverse primer 5'-GTGAGGTTCTGGTTGTAGCTGACA-3'
− TaqMan probe 5'-FAM-TCACGGTGGTTGCCATCACTGTCTTC-TAMRA-3'
−
−
−
−
−Primers chosen for experiment (according to Andre's advice)
−
−(designed using [http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi PrimerPlus3]
−
−
−
−NR1 U11418.1
−2360 Forward ttcacagaagtgcgatctgg, Tm 60.0
−2467 Reverse atggacagggaaacgttctg, Tm 60.0
−Product size of 108 bp
−
−
−Serine Racemase NM_198757
−
−856 forward: tggtagatgcgttggtggta, Tm 60.0
−958 reverse: tcagcagcgtacaccttcac, Tm60.1
−
−674-807, product size 134bp
−
−Rat GAPDH
−
−Forward TCACTGCCACTCAGAAGACTG
−Reverse GGATGACCTTGCCCACAGC
Primers were ordered 2009 May from Sigma Aldrich
Primers were ordered 2009 May from Sigma Aldrich
+
+(designed using [http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi PrimerPlus3])
+
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=Primers for Real-Time PCR=
=Primers for Real-Time PCR=
−Looked at primers from Yamamoto et al. 2008 PMID 17957211 using Applied Biosystems, but ended up designing own primers above.
−[[Category:Taste_Aversion]]
[[Category:Taste_Aversion]]