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Line 34: − Taken from Yamamoto et al. 2008 PMID 17957211 using Applied Biosystems+ − − − NR1 − Forward primer 5'-GTTCTTCCGCTCAGGCTTTG-3' − Reverse primer 5'-AGGGAAACGTTCTGCTTCCA-3' − TaqMan probe 5'-FAM-CGGCATGCGCAAGGACAGCC-TAMRA-3' − − NR2A − Forward primer 5'-AGCCCCCTTCGTCATCGTA-3' − Reverse primer 5'-GACAGGGCACCGTGTTCCT-3' − TaqMan probe 5'-FAM-AGGACATAGACCCCCTGACTGAGACCTGTG-TAMRA-3' − − NR2B − Forward primer 5'-CCCCCAAGTTCTGGTTGGT-3' − Reverse primer 5'-TTTTGGGAACGAGCTTTGCT-3' − TaqMan probe 5'-FAM-TTGGCCGTCTTGGCCGTATCAGG-TAMRA-3' − − NR2C − Forward primer 5'-GGGTGATGATGTTCGTGATGTG-3' − Reverse primer 5'-GTGAGGTTCTGGTTGTAGCTGACA-3' − TaqMan probe 5'-FAM-TCACGGTGGTTGCCATCACTGTCTTC-TAMRA-3' − − − − − Primers chosen for experiment (according to Andre's advice) − − (designed using [http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi PrimerPlus3] − − − − NR1 U11418.1 − 2360 Forward ttcacagaagtgcgatctgg, Tm 60.0 − 2467 Reverse atggacagggaaacgttctg, Tm 60.0 − Product size of 108 bp − − − Serine Racemase NM_198757 − − 856 forward: tggtagatgcgttggtggta, Tm 60.0 − 958 reverse: tcagcagcgtacaccttcac, Tm60.1 − − 674-807, product size 134bp − − Rat GAPDH − − Forward TCACTGCCACTCAGAAGACTG − Reverse GGATGACCTTGCCCACAGC
cleaned up
Primers were ordered 2009 May from Sigma Aldrich
Primers were ordered 2009 May from Sigma Aldrich
(designed using [http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi PrimerPlus3])
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=Primers for Real-Time PCR=
=Primers for Real-Time PCR=
Looked at primers from Yamamoto et al. 2008 PMID 17957211 using Applied Biosystems, but ended up designing own primers above.
[[Category:Taste_Aversion]]
[[Category:Taste_Aversion]]