Difference between revisions of "TN Unconditioned Aversions in 14-3-3 Transgenics"

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Continuation of  [[TK CTA in 14-3-3 Transgenics]] and [[TL Stronger CTA in 14-3-3 Transgenics]] and [[TM Stronger NaCl CTA in 14-3-3 Transgenics]].
  
Continuation of [[TM Stronger NaCl CTA in 14-3-3 Transgenics]]
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Tests of unconditioned aversions to bitter, salt, or sour tastants
  
Tests of unconditioned aversions to bitter, salt, or sour tastants
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==Quinine Sulfate==
  
 
30 micromolar quinine sulfate (MW  391.47)
 
30 micromolar quinine sulfate (MW  391.47)
  
1000x = 30 mM quinine sulfate  = 0.03 * 392 g / liter = 11.76 g / liter = 5.88g / 500 ml
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50x = 1.5 mM quinine sulfate  = 0.0015 * 392 g / liter = 0.588 g / liter = 0.3g / 500 ml (note: solubility of quinine sulfate is only 0.12g/100ml)
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40 ml of  50x + 2 liters -> 30 micromolar quinine sulfate
  
2 ml of 1000x + 2 liters -> 30 micromolar quinine sulfate
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==NaCl==
  
 
600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters
 
600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters
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==HCl==
  
 
0.01 M HCl (MW 36.5, comes in 10M solution)
 
0.01 M HCl (MW 36.5, comes in 10M solution)

Latest revision as of 12:37, 20 March 2012

Continuation of TK CTA in 14-3-3 Transgenics and TL Stronger CTA in 14-3-3 Transgenics and TM Stronger NaCl CTA in 14-3-3 Transgenics.

Tests of unconditioned aversions to bitter, salt, or sour tastants

Quinine Sulfate

30 micromolar quinine sulfate (MW 391.47)

50x = 1.5 mM quinine sulfate = 0.0015 * 392 g / liter = 0.588 g / liter = 0.3g / 500 ml (note: solubility of quinine sulfate is only 0.12g/100ml)

40 ml of 50x + 2 liters -> 30 micromolar quinine sulfate

NaCl

600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters

HCl

0.01 M HCl (MW 36.5, comes in 10M solution)

2 ml of 10M + 2 liters -> 0.01 M HCl