Difference between revisions of "TN Unconditioned Aversions in 14-3-3 Transgenics"
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+ | Continuation of [[TK CTA in 14-3-3 Transgenics]] and [[TL Stronger CTA in 14-3-3 Transgenics]] and [[TM Stronger NaCl CTA in 14-3-3 Transgenics]]. | ||
− | + | Tests of unconditioned aversions to bitter, salt, or sour tastants | |
− | + | ==Quinine Sulfate== | |
30 micromolar quinine sulfate (MW 391.47) | 30 micromolar quinine sulfate (MW 391.47) | ||
− | + | 50x = 1.5 mM quinine sulfate = 0.0015 * 392 g / liter = 0.588 g / liter = 0.3g / 500 ml (note: solubility of quinine sulfate is only 0.12g/100ml) | |
+ | |||
+ | 40 ml of 50x + 2 liters -> 30 micromolar quinine sulfate | ||
− | + | ==NaCl== | |
600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters | 600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters | ||
+ | |||
+ | ==HCl== | ||
0.01 M HCl (MW 36.5, comes in 10M solution) | 0.01 M HCl (MW 36.5, comes in 10M solution) |
Latest revision as of 12:37, 20 March 2012
Continuation of TK CTA in 14-3-3 Transgenics and TL Stronger CTA in 14-3-3 Transgenics and TM Stronger NaCl CTA in 14-3-3 Transgenics.
Tests of unconditioned aversions to bitter, salt, or sour tastants
Quinine Sulfate
30 micromolar quinine sulfate (MW 391.47)
50x = 1.5 mM quinine sulfate = 0.0015 * 392 g / liter = 0.588 g / liter = 0.3g / 500 ml (note: solubility of quinine sulfate is only 0.12g/100ml)
40 ml of 50x + 2 liters -> 30 micromolar quinine sulfate
NaCl
600 mM NaCl (MW 60) = 36 g / liter = 72 g/ 2 liters
HCl
0.01 M HCl (MW 36.5, comes in 10M solution)
2 ml of 10M + 2 liters -> 0.01 M HCl