Difference between revisions of "TK CTA in 14-3-3 Transgenics"

Latest revision as of 14:14, 8 May 2012

Test of CTA in transgenic mice that overexpress R18 peptide, which blocks the ability of 14-3-3 to bind other proteins. Zhou's founder Line 132.

1-23-12 rev. 2012-2-7

Adam Kimbrough, Yuying Wu, Dr. Thomas A. Houpt, Dr. Yi Zhou

TK1-31 (1-8=mutant LiCl, 9-16= mutant NaCl, 17-24= wildtype LiCl, 25-31= wildtype NaCl)

8 wildtype females 7 wildtype males

8 mutant females 8 mutant males

LiCl mutant group TK 1-4 =mutant females TK 5-8 =mutant males

NaCl mutant group TK 9-12 = mutant females TK 13-16= mutant males

LiCl wildtype group TK 17-20 = wildtype females TK 21-24 = wildtype males

NaCl wildtype group TK 25-28 = wildtype females TK 29-31 = wildtype male

6 Days of water deprivation (3h, 3h, 1h, 1h, 30m, 30m -> conditioning 10m) with intakes recorded each day.

Conditioning Day: 2012-2-7

10 minute 0.125% saccharin access (2g/liter; 4g/2 liters)

10 minutes after saccharin access injection with 0.15M LiCl or NaCl (20 ml/kg-intermediate mouse dose)

Intakes recorded

6 days 24-h 2 bottle access to 0.125% saccharin and water with intakes recorded.

@@ Line 1: / Line 1: @@
-Test of CTA in transgenic mice that overexpress R18 peptide, which blocks the ability of [[14-3-3]] to bind other proteins.
+Test of CTA in transgenic mice that overexpress R18 peptide, which blocks the ability of [[14-3-3]] to bind other proteins. Zhou's founder Line 132.
+Continued in experiment   [[TL Stronger CTA in 14-3-3 Transgenics]] and [[TM Stronger NaCl CTA in 14-3-3 Transgenics]] and [[TN Unconditioned Aversions in 14-3-3 Transgenics]].
 -23-12 rev. 2012-2-7