Difference between revisions of "TK CTA in 14-3-3 Transgenics"
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| + | Test of CTA in transgenic mice that overexpress R18 peptide, which blocks the ability of [[14-3-3]] to bind other proteins. Zhou's founder Line 132. | ||
| − | + | Continued in experiment [[TL Stronger CTA in 14-3-3 Transgenics]] and [[TM Stronger NaCl CTA in 14-3-3 Transgenics]] and [[TN Unconditioned Aversions in 14-3-3 Transgenics]]. | |
1-23-12 rev. 2012-2-7 | 1-23-12 rev. 2012-2-7 | ||
| − | Adam Kimbrough | + | Adam Kimbrough, Yuying Wu, Dr. Thomas A. Houpt, Dr. Yi Zhou |
| − | Dr. Thomas A. Houpt | ||
| − | Dr. Yi Zhou | ||
TK1-31 (1-8=mutant LiCl, 9-16= mutant NaCl, 17-24= wildtype LiCl, 25-31= wildtype NaCl) | TK1-31 (1-8=mutant LiCl, 9-16= mutant NaCl, 17-24= wildtype LiCl, 25-31= wildtype NaCl) | ||
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6 Days of water deprivation (3h, 3h, 1h, 1h, 30m, 30m -> conditioning 10m) with intakes recorded each day. | 6 Days of water deprivation (3h, 3h, 1h, 1h, 30m, 30m -> conditioning 10m) with intakes recorded each day. | ||
| + | |||
| + | Conditioning Day: 2012-2-7 | ||
10 minute 0.125% saccharin access (2g/liter; 4g/2 liters) | 10 minute 0.125% saccharin access (2g/liter; 4g/2 liters) | ||
| Line 41: | Line 42: | ||
Intakes recorded | Intakes recorded | ||
| − | + | 6 days 24-h 2 bottle access to 0.125% saccharin and water with intakes recorded. | |
| + | |||
| + | Experiment continued as [[TL Stronger CTA in 14-3-3 Transgenics]] | ||
[[Category: CTA Experiments]] | [[Category: CTA Experiments]] | ||
Latest revision as of 15:14, 8 May 2012
Test of CTA in transgenic mice that overexpress R18 peptide, which blocks the ability of 14-3-3 to bind other proteins. Zhou's founder Line 132.
Continued in experiment TL Stronger CTA in 14-3-3 Transgenics and TM Stronger NaCl CTA in 14-3-3 Transgenics and TN Unconditioned Aversions in 14-3-3 Transgenics.
1-23-12 rev. 2012-2-7
Adam Kimbrough, Yuying Wu, Dr. Thomas A. Houpt, Dr. Yi Zhou
TK1-31 (1-8=mutant LiCl, 9-16= mutant NaCl, 17-24= wildtype LiCl, 25-31= wildtype NaCl)
8 wildtype females 7 wildtype males
8 mutant females 8 mutant males
LiCl mutant group TK 1-4 =mutant females TK 5-8 =mutant males
NaCl mutant group TK 9-12 = mutant females TK 13-16= mutant males
LiCl wildtype group TK 17-20 = wildtype females TK 21-24 = wildtype males
NaCl wildtype group TK 25-28 = wildtype females TK 29-31 = wildtype male
6 Days of water deprivation (3h, 3h, 1h, 1h, 30m, 30m -> conditioning 10m) with intakes recorded each day.
Conditioning Day: 2012-2-7
10 minute 0.125% saccharin access (2g/liter; 4g/2 liters)
10 minutes after saccharin access injection with 0.15M LiCl or NaCl (20 ml/kg-intermediate mouse dose)
Intakes recorded
6 days 24-h 2 bottle access to 0.125% saccharin and water with intakes recorded.
Experiment continued as TL Stronger CTA in 14-3-3 Transgenics